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	Provedor de dados Genet. Mol. Biol. (2) Autor Ceresini,Paulo C. (2) Ciampi,Maisa B. (1) Costa-Souza,Elaine (1) Furtado,Edson L. (1) Gale,Liane Rosewich (1) Lemos,Eliana G. de Macedo (1) Souza,Nilton L. (1) Zala,Marcello (1) Mais... Palavra-chave Allelic discrimination (1) Ecological speciation (1) Fungal plant pathogens (1) Host specialization (1) Multilocus genotyping (1) Phylogenetics (1) Polymorphisms (1) Population divergence (1) Primer design (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2009 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Distinctively variable sequence-based nuclear DNA markers for multilocus phylogeography of the soybean- and rice-infecting fungal pathogen Rhizoctonia solani AG-1 IA Genet. Mol. Biol. Ciampi,Maisa B.; Gale,Liane Rosewich; Lemos,Eliana G. de Macedo; Ceresini,Paulo C.. A series of multilocus sequence-based nuclear DNA markers was developed to infer the phylogeographical history of the Basidiomycetous fungal pathogen Rhizoctonia solani AG-1 IA infecting rice and soybean worldwide. The strategy was based on sequencing of cloned genomic DNA fragments (previously used as RFLP probes) and subsequent screening of fungal isolates to detect single nucleotide polymorphisms (SNPs). Ten primer pairs were designed based on these sequences, which resulted in PCR amplification of 200-320 bp size products and polymorphic sequences in all markers analyzed. By direct sequencing we identified both homokaryon and heterokaryon (i.e. dikaryon) isolates at each marker. Cloning the PCR products effectively estimated the allelic phase from... Tipo: Info:eu-repo/semantics/article Palavras-chave: Multilocus genotyping; Polymorphisms; Allelic discrimination; Primer design. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400027 Evidence that the Ceratobasidium-like white-thread blight and black rot fungal pathogens from persimmon and tea crops in the Brazilian Atlantic Forest agroecosystem are two distinct phylospecies Genet. Mol. Biol. Ceresini,Paulo C.; Costa-Souza,Elaine; Zala,Marcello; Furtado,Edson L.; Souza,Nilton L.. The white-thread blight and black rot (WTBR) caused by basidiomycetous fungi of the genus Ceratobasidium is emerging as an important plant disease in Brazil, particularly for crop species in the Ericales such as persimmon (Diospyros kaki) and tea (Camellia sinensis). However, the species identity of the fungal pathogen associated with either of these hosts is still unclear. In this work, we used sequence variation in the internal transcribed spacer regions, including the 5.8S coding region of rDNA (ITS-5.8S rDNA), to determine the phylogenetic placement of the local white-thread-blight-associated populations of Ceratobasidium sp. from persimmon and tea, in relation to Ceratobasidium species already described world-wide. The two sister populations of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ecological speciation; Fungal plant pathogens; Host specialization; Population divergence; Phylogenetics. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300016 Registros recuperados: 2 Primeira ... 1 ... Última

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